This Mouse Fibroblast Growth Factor Receptor 4 (FGFR4) ELISA Kit from Innovative Research is intended for quantitative detection of mouse FGFR4 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This mouse FGFR4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FGFR4 has been precoated onto 96-well plates. Standards(Expression system for standard: , Immunogen sequence: ) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGFR4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FGFR4 amount of sample captured in plate.
- Detection Target: Fibroblast Growth Factor Receptor 4 (FGFR4)
- Uniprot ID: )
- Reactivity: Mouse
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 312pg/ml-20000pg/ml
- Sensitivity: <50pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat.
